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crisp_mm_plugin [2018/11/20 16:48]
melissa updating to v2.02 first draft
crisp_mm_plugin [2018/11/21 10:02] (current)
melissa changed pic for Lat. Adj. Knob; added figure number and caption
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-[{{ ::​micromanager_crisp.gif?​direct |Fig. 1\\ **Access the Plugin window**\\ Micro-Manager Window > **Plugins** > **Beta**}}]+[{{ ::​micromanager_crisp.gif?​direct |Fig. 1\\ **Access the Plugin window**\\ Micro-Manager Window > **Plugins** > **Beta** }}]
  
 ===== Plugin Controls Explained ===== ===== Plugin Controls Explained =====
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   * Check the SNR display. If <2 dB, click through the rest of the 4-step calibration,​ increase the LED Intensity, and begin the calibration again. If SNR is still low after the LED is at maximum intensity, proceed to the next step anyway.   * Check the SNR display. If <2 dB, click through the rest of the 4-step calibration,​ increase the LED Intensity, and begin the calibration again. If SNR is still low after the LED is at maximum intensity, proceed to the next step anyway.
   * Click the **3) DITHER Button**.   * Click the **3) DITHER Button**.
-  * Check the Error #. It must be at least +/- 100; if it isn’t, move the Lateral Adjustment ​screw. The farther from zero the error number, the stronger the lock.+  * Check the Error #. It must be at least +/- 100; if it isn’t, move the Lateral Adjustment ​Screw. The farther from zero the error number, the stronger the lock.
   * Press the **4) Set Gain** button.   * Press the **4) Set Gain** button.
   * Press the **Lock** button to preserve the focal position. (Press the **Unlock** button to release CRISP'​s focus lock.)   * Press the **Lock** button to preserve the focal position. (Press the **Unlock** button to release CRISP'​s focus lock.)
   * If CRISP loses lock, repeat the calibration steps and try to get a higher error number after dithering.   * If CRISP loses lock, repeat the calibration steps and try to get a higher error number after dithering.
  
-{{ crisp_mm_1_.gif?300 |}}+[{{ ::​crisplateraladjknob.png?300 |Fig. 3\\ **Lateral Adjustment Knob**\\ Used to increase error number during dither}}]
    
 ===== Additional Tips ===== ===== Additional Tips =====
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 Further Reading Further Reading
-Refer to [[crisp_manual|CRISP : Continuous Autofocus System]] for a better ​description of CRISP operation and troubleshooting guides.+Refer to [[crisp_manual|CRISP : Continuous Autofocus System]] for a description of CRISP operation and troubleshooting guides.
  
 {{tag>​crisp tech_note MicroManager Micro-Manager}} {{tag>​crisp tech_note MicroManager Micro-Manager}}
crisp_mm_plugin.txt · Last modified: 2018/11/21 10:02 by melissa