User Tools

Site Tools



This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
crisp_mm_plugin [2018/11/21 00:48]
melissa updating to v2.02 first draft
crisp_mm_plugin [2019/04/18 23:34] (current)
Line 1: Line 1:
 +====== Micro-Manager’s CRISP Control Plugin ======
 +===== Accessing the Plugin =====
 +The Plugin comes default in current versions Micro-Manager;​ if you don't have it in **Plugins** > **Beta**, update Micro-Manager to the [[https://​​wiki/​Micro-Manager_Nightly_Builds|latest nightly build]]. ​
 +[{{ ::​micromanager_crisp.gif?​direct |Fig. 1\\ **Access the Plugin window**\\ Micro-Manager Window > **Plugins** > **Beta** }}]
 +===== Plugin Controls Explained =====
 +[{{ ::​mm_crisp2.02marked.png?​400 |Fig. 2\\ **CRISP Plugin'​s control window**\\ The red rectangle indicates which axis the Plugin is controlling.}}]
 +=== Buttons ===
 +  * **1) IDLE**: turns off the IR LED and stops position adjustments
 +  * **2) LOG CAL**: puts the CRISP in Log Amp Calibration state and measures SNR
 +  * **3) DITHER**: causes CRISP to dither in order to measure the Error #
 +  * **4) SET GAIN**: puts the CRISP in Set Gain state
 +  * **Reset Offsets**: resets the focus offset to zero for the present position
 +  * **Lock**: sets the focal position
 +  * **Unlock**: unlocks the focal position
 +  * **Save**: saves the settings (for all of the spin boxes) onto the controller
 +=== Spin Boxes ===
 +  * **LED Intensity**:​ controls CRISP’s infrared (IR) LED from 0-100%
 +  * **Gain**: controls the gain multiplier or loop gain; decrease the value if CRISP seems to oscillate or jitter
 +  * **Avg**: the number of samples to be averaged
 +  * **Obj NA**: the objective’s numerical aperture
 +=== Check Box ===
 +  * **Polling**:​ directs the Plugin to poll or query the controller at frequent intervals for CRISP state, error number, and SNR; uncheck when done with the 4-step calibration
 +=== Reported Values ===
 +  * **CRISP State**: such as Idle, Calibrating,​ Ready, In Focus, etc.
 +  * **Error #**: during DITHER higher error values are better; after SET GAIN the Error # will be close to zero
 +  * **SNR**: (in decibels) should be at least 2 dB else CRISP performance may be unstable and lock will be lost easily; increasing LED intensity and performing the 4-step calibration again to increase the SNR
 +  * **AGC**: minimum of ~20 is acceptable for average samples; may be much higher for very reflective samples
 +  * **Sum**: indicates the amount of light hitting the photodiode; if outside 50-80, redo the 4-step calibration
 +  * **Offset**: the difference between the locked-in focal position and the current position
 +===== Quick Setup Guide =====
 +  * Start the 4-step calibration((Note that the 4-step calibration should always go through all 4 steps before re-starting;​ Log Amp Calibration has a countdown in the CRISP State display but otherwise you can quickly click through them to get back to the beginning.)) by clicking the **1) Idle** button. ​
 +  * Enter the objective’s NA (which affects the dither).
 +  * Click the **2) LOG CAL** button.
 +  * Check the SNR display. If <2 dB, click through the rest of the 4-step calibration,​ increase the LED Intensity, and begin the calibration again. If SNR is still low after the LED is at maximum intensity, proceed to the next step anyway.
 +  * Click the **3) DITHER Button**.
 +  * Check the Error #. It must be at least +/- 100; if it isn’t, move the Lateral Adjustment Screw. The farther from zero the error number, the stronger the lock.
 +  * Press the **4) Set Gain** button.
 +  * Press the **Lock** button to preserve the focal position. (Press the **Unlock** button to release CRISP'​s focus lock.)
 +  * If CRISP loses lock, repeat the calibration steps and try to get a higher error number after dithering.
 +[{{ ::​crisplateraladjknob.png?​300 |Fig. 3\\ **Lateral Adjustment Knob**\\ Used to increase error number during dither}}]
 +===== Additional Tips =====
 +If you would like CRISP to maintain focus at a certain position, but the error isn’t 0, use the **Reset Offsets** button to make your desired focal position have “0” error.
 +Further Reading
 +Refer to [[crisp_manual|CRISP : Continuous Autofocus System]] for a description of CRISP operation and troubleshooting guides.
 +{{tag>​crisp tech_note MicroManager Micro-Manager}}
Address: 29391 W Enid Rd. Eugene, OR 97402, USA | Phone: +1 (541) 461-8181
crisp_mm_plugin.txt · Last modified: 2019/04/18 23:34 (external edit)