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mim_ramm_vts [2019/08/21 18:52]
jon [MIM Tube Lenses and Assemblies]
mim_ramm_vts [2019/10/03 00:47] (current)
jon added Tube-358 and made Tube-180L more prominent
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 With infinity microscope systems, the objective can be spaced away from the tube lens without changing the optical magnification. ​ This “infinity space” provides a region where other optical systems can be coupled to the microscope relatively easily. ​ For epi-fluorescent illumination,​ a filter cube with a dichroic beam splitter can be added to provide the illumination path.  ​ With infinity microscope systems, the objective can be spaced away from the tube lens without changing the optical magnification. ​ This “infinity space” provides a region where other optical systems can be coupled to the microscope relatively easily. ​ For epi-fluorescent illumination,​ a filter cube with a dichroic beam splitter can be added to provide the illumination path.  ​
  
-For best imaging performance we recommend the ''​C60_Tube_B''​ which uses a Nikon 200mm multi-element tube lens.+For best imaging performance we recommend the ''​C60-Tube-B''​ which uses a Nikon 200mm multi-element tube lens or else (as of 2019) the ''​C60-180-L''​ which uses the Olympus 180mm multi-element tube lens.
  
 [{{ mim_ramm_5_.jpg?​300 | C60-Tube-B}}] [{{ mim_ramm_5_.jpg?​300 | C60-Tube-B}}]
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 [{{ :​tube_lens_figures.JPG?​direct&​300 | Click to Expand}}] [{{ :​tube_lens_figures.JPG?​direct&​300 | Click to Expand}}]
  
-In addition to the standard C60-TUBE-B, there are also several ​other options for tubes and tube lenses that can be used to obtain different final magnifications. ​   The achromatic lens tubes, Tube-100, Tube-200, etc., include the male ring end built into the assembly. ​ A coupling C60-RING is required for Tube-B and Tube-Z13.+In addition to the standard C60-TUBE-B, there are also many other options for tubes and tube lenses that can be used to obtain different final magnifications. ​ The achromatic lens tubes, Tube-100, Tube-200, etc., include the male ring end built into the assembly. ​ A coupling C60-RING is required for Tube-B, Tube-180L, ​and Tube-Z13.
  
 The distance from the end of the tube to the image plane is 60 mm for all tubes. ​ The tubes have a 50mm O.D. and are terminated with a 30mm I.D. flange. ​ The table below lists other tube lens options and specifications for the various tube lens assemblies with dimensions as indicated in Figure 1. The distance from the end of the tube to the image plane is 60 mm for all tubes. ​ The tubes have a 50mm O.D. and are terminated with a 30mm I.D. flange. ​ The table below lists other tube lens options and specifications for the various tube lens assemblies with dimensions as indicated in Figure 1.
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 ^ C60-TUBE-265D ​ | 265             | 1.33              | 1.47                | Dual Achromat ​                                                    | ~250      | 229    | ^ C60-TUBE-265D ​ | 265             | 1.33              | 1.47                | Dual Achromat ​                                                    | ~250      | 229    |
 ^ C60-TUBE-300 ​  | 300             | 1.50              | 1.67                | Achromat ​                                                         | ~291      | 252.0    | ^ C60-TUBE-300 ​  | 300             | 1.50              | 1.67                | Achromat ​                                                         | ~291      | 252.0    |
 +^ C60-TUBE-358 ​  | 358             | 1.79              | 1.99                | Achromat ​                                                         | ~355?      | 252.0    |
 ^ C60-TUBE-400 ​  | 400             | 2.00              | 2.22                | Achromat ​                                                         | ~391      | 352.0    | ^ C60-TUBE-400 ​  | 400             | 2.00              | 2.22                | Achromat ​                                                         | ~391      | 352.0    |
  
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 Spherical aberrations are usually the main determinant of tube lens performance. ​ This quantifies the degree to which all parallel rays are focused to the same point axially. ​ Chromatic aberrations are also important for applications where more than one wavelength is used (e.g. fluorescent samples with both GFP and mCherry labels). Spherical aberrations are usually the main determinant of tube lens performance. ​ This quantifies the degree to which all parallel rays are focused to the same point axially. ​ Chromatic aberrations are also important for applications where more than one wavelength is used (e.g. fluorescent samples with both GFP and mCherry labels).
  
-All ASI's tube lenses are intended for use with infinity-corrected objectives. ​ Nikon and Olympus ​objectives ​(and tube lenses) include all corrections internally and can be safely mixed and matched for convenience,​ to adjust the magnification,​ etc.  Leica and Zeiss objectives and tube lenses split the chromatic correction between the objective and tube lens in a proprietary way and cannot be mixed without some performance degradation.+All ASI's tube lenses are intended for use with infinity-corrected objectives. ​ Nikon and Olympus ​objective lenses ​(and tube lenses) include all corrections internally and can be safely mixed and matched for convenience,​ to adjust the magnification,​ etc.  Leica and Zeiss objectives and tube lenses split the chromatic correction between the objective and tube lens in a proprietary way and cannot be mixed without some performance degradation.
  
-For best imaging performance we recommend the ''​C60_Tube_B''​ which uses a Nikon 200 mm focal length multi-element tube lens.  In general longer focal length lenses will have better performance. ​ By using multi-element lenses ''​C60_TUBE_180D'',​ ''​C60_TUBE_140D'',​ ''​C60_TUBE_125D'',​ and ''​C60_TUBE_100D''​ usually are satisfactory for imaging. ​ Likewise ''​C60_TUBE_250'',​ ''​C60_TUBE_300'',​ and ''​C60_TUBE_400''​ are usually satisfactory for imaging even though they only contain a single ​element.  ''​C60_TUBE_100''​ and ''​C60_TUBE_70''​ are not recommended for imaging applications,​ and ''​C60_TUBE_160''​ is marginal for imaging applications.+For best imaging performance we recommend ​either ​the ''​C60-Tube-B''​ which uses a Nikon 200 mm focal length multi-element tube lens or the Olympus equivalent ''​C60-Tube-180L''​.  In general longer focal length lenses will have better performance, and smaller objective pupils are more forgiving.  By using multi-element lenses ''​C60-TUBE-180D'',​ ''​C60-TUBE-140D'',​ ''​C60-TUBE-125D'',​ and ''​C60-TUBE-100D''​ usually are satisfactory for imaging. ​ Likewise ''​C60-TUBE-250'',​ ''​C60-TUBE-265'',​ ''​C60-TUBE-300'',​ ''​C60-TUBE-358'',​ and ''​C60-TUBE-400''​ are usually satisfactory for imaging even though they only contain a single ​doublet.  ''​C60-TUBE-100''​ and ''​C60-TUBE-70''​ are not recommended for imaging applications,​ and ''​C60-TUBE-160''​ is marginal for imaging applications.
  
  
 ==== Example Images ==== ==== Example Images ====
  
-Imaging performance of the tube lens will depend upon the suitable combination of lens and objective, the field of view in the image, and the focal length of the tube lens.  In general, longer focal length lenses will have better performance across the field of view.  To illustrate this, we show some sample images taken with a variety of tube-lenses using a high quality objectives. ​ All of the images are of a fixed stained bone marrow smear, which provides a nice uniform large area color sample with large spatial dynamic range. ​ The images show a reference circle corresponding to a 25mm field number (FN) for the objective. ​ Magnified sections of the image in the center of the field and near the objective design FN are shown as well.  Images were taken using a Sony NEX-3 camera with 23.4 mm x 15.6 mm sensor with 5.1µ pixels. ​ For a more complete set of test images, see the Tube Lens Performance document.+Imaging performance of the tube lens will depend upon the suitable combination of lens and objective, the pupil size of the objective, the field of view in the image, and the focal length of the tube lens.  In general, longer focal length lenses will have better performance across the field of view.  To illustrate this, we show some sample images taken with a variety of tube-lenses using a high quality objectives. ​ All of the images are of a fixed stained bone marrow smear, which provides a nice uniform large area color sample with large spatial dynamic range. ​ The images show a reference circle corresponding to a 25mm field number (FN) for the objective. ​ Magnified sections of the image in the center of the field and near the objective design FN are shown as well.  Images were taken using a Sony NEX-3 camera with 23.4 mm x 15.6 mm sensor with 5.1µ pixels. ​ For a more complete set of test images, see the Tube Lens Performance document.
  
 == C60-Tube_B and Nikon 20X 0.75 Plan Apo Objective == == C60-Tube_B and Nikon 20X 0.75 Plan Apo Objective ==
Address: 29391 W Enid Rd. Eugene, OR 97402, USA | Phone: +1 (541) 461-8181
mim_ramm_vts.txt · Last modified: 2019/10/03 00:47 by jon