synchronized_zaxis_focus_sweeps
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+ | ====== Synchronized Z-axis Focus Sweeps ====== | ||
+ | Focus sweeps provide a simple way to integrate many focal planes in a single image. | ||
+ | |||
+ | ===== Required Configuration ===== | ||
+ | |||
+ | The following components are required: | ||
+ | * MS2000WK controller with firmware** SCAN MODULE** | ||
+ | * Mechanical Z-axis Drive | ||
+ | * Camera with TTL gated exposure mode | ||
+ | |||
+ | ===== Procedure ===== | ||
+ | |||
+ | The user should familiarize themselves with the [[commands: | ||
+ | |||
+ | As an example, let us assume that we would like to take a 200ms exposure over a swept focal range of 10μm. | ||
+ | |||
+ | \begin{equation} Speed = \frac{Focal Range}{Exposure Time} \\ | ||
+ | |||
+ | Speed = \frac{0.010 mm}{0.200 sec} = 0.05 mm/sec \end{equation} | ||
+ | |||
+ | The following commands will set up the single Z-axis scan at the correct speed: | ||
+ | |||
+ | '' | ||
+ | |||
+ | '' | ||
+ | |||
+ | '' | ||
+ | |||
+ | '' | ||
+ | |||
+ | '' | ||
+ | |||
+ | At this point the controller will begin successive scans of z-axis followed by rapid retraces. | ||
+ | |||
+ | - Use TTL OUT0 (internal SV1-2) and the command [[commands: | ||
+ | - Use the SCAN SYNC (internal SV1-7) output directly. | ||
+ | |||
+ | With either (or both) method(s), you should be able to display the TTL trigger pulses on an oscilloscope. | ||
+ | |||
+ | There may be some reason you wish to pause between successive sweeps. | ||
+ | |||
+ | The basic setup parameters for your sweep can be stored into nonvolatile memory in the controller using the command [[commands: | ||
+ | |||
+ | A scan can be initiated, stopped, and restarted just by using the [[commands: | ||
+ | |||
+ | ===== Overshoot Parameter ===== | ||
+ | |||
+ | The overshoot parameter is set using [[commands: | ||
+ | |||
+ | |||
+ | {{tag> |
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