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ultimate_resolution_of_microscopes [2019/02/25 12:33]
jon
ultimate_resolution_of_microscopes [2019/02/25 12:37] (current)
jon
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 where $λ$ is the wavelength of the light and $\mathrm{NA}_{obj}$ is the numerical objective of the imaging objective lens.  The prefactor varies with the criteria to define resolution, e.g. commonly a prefactor of 0.5 is used instead of 0.61.  Lateral resolution is synonymous with $d_{xy}$. where $λ$ is the wavelength of the light and $\mathrm{NA}_{obj}$ is the numerical objective of the imaging objective lens.  The prefactor varies with the criteria to define resolution, e.g. commonly a prefactor of 0.5 is used instead of 0.61.  Lateral resolution is synonymous with $d_{xy}$.
  
-For transmitted light microscopy, the resolving power is also affected by the numerical aperture of the illumination optics, in this case it is given by +For transmitted light microscopy, the resolving power is also affected by the numerical aperture of the illumination optics.((A similar situation occurs in confocal microscopy where the same numerical aperture appears on both the illumination and detection pathsbut it only applies ​in the case of an in the limit of an infinitesimally small pinhole.)) ​ For transmitted light using a condenser with numerical aperture $NA_{cond}$ the lateral resolution ​is given by 
  
 \begin{equation} \begin{equation}
ultimate_resolution_of_microscopes.txt · Last modified: 2019/02/25 12:37 by jon