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Add CRISP to a MIM1

In order to add a CRISP autofocus device to the simplest form of ASI's inverted Modular Infinity Microscope1), an additional optical path must be installed on the microscope, a control card must be inserted into the Tiger controller and plugged into the card of the axis it will control (typically the Z-axis), and the firmware of that card must be updated.

An optical path for CRISP

  1. Remove the Prism Block from the LS-50 (Z-stage) body.
    1. Move the epi-illumination assembly.
      1. Loosen the three set screws connecting the epi-illumination assembly (Tube/Condenser lens, Filter Wheel, Liquid Light Guide adapter) to the CUBE III, requires 2 mm hex driver.
      2. Lightly loosen the two screws in the ring clamp supporting the epi-illumination assembly, requires 2 mm hex driver.
      3. Slide (and jiggling a bit helps too) the epi-illumination path assembly away from the CUBE III.
    2. Move the imaging assembly back.
      1. Loosen the three set screws in the Prism Block (Figs 1 & 2: green); requires 2 mm hex driver.
      2. Lightly loosen the two screws in the ring clamp supporting the imaging path assembly; requires 2.5 mm hex driver. (The imaging assembly includes: CUBE III, polarizer slider, Tube lens “B”, C-mount adapter, and camera). (Tip: Do not loosen the screws with 5 mm hex heads in the drop arms.)
      3. Remove the camera if it is currently mounted.
      4. Slide the imaging path assembly away from the Prism Block by a few centimeters.
    3. Remove the four bolts (Fig 2: pink) to finally remove the Prism Block, requires 5 mm hex driver.
  2. Mount the 2nd Port and Prism block.
    1. Mount the 2nd Port (Figs 2: orange) in the position previously occupied by the Prism Block, requires 5 mm hex driver.
    2. Mount the Prism Block (Fig 2: pink) directly under the 2nd Port’s coupling tube.
  3. Replace the imaging assembly and epi-illumination assembly.
    1. Slide the imaging assembly up to the 2nd Port; tighten at least 3 set screws on the 2nd Port (Fig 2: blue), requires 2 mm hex driver.
    2. Slide the epi-illumination path up to the CUBE III; tighten the 3 set screws that secure them together.

Adjust the detector position for optimum performance

1)Click Step 2 “Dither” state. Adjust the main CRISP adjusting Thumb Screw for maximum ERR number. 2)Click Step 3 “Set Gain” and wait a few seconds for it to finish. 3)Re-focus microscope on sample and set the Z-axis controller position to Zero. (HERE Z=0) 4)Be sure NA setting is correct for the objective and click “GRAPH” button.

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Figure 5

5)Choose and operating point and adjust the main CRISP adjusting Thumbscrew to get there. If you only adjust for maximum ERR number, then the point you choose will be at the steepest part of the focus curve. Sometimes this can be very close to where the curve makes a sudden change in slope.

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The MPPI-3 Milli Pulse Pressure Injector, has been designed and tested to provide years of reliable service.

In order to insure that the unit operates as designed. use only nitrogen or clean dry compressed air as the pressure source.

The design of the MPPI-3 requires little maintenance on the part of the user. In fact, there are only two areas that the user needs to be aware. The first is to periodically check for moisture and other contaminants in the pressure source. The internal in-line filter that protrudes from the back of the unit has a small needle valve located on the bottom. This valve should be depressed periodically to check for moisture from the pressure source. If excessive moisture is noted, the pressure source should be changed. In the event that the filter becomes degraded or plugged, contact ASI for a replacement.

The second area of maintenance that the user should be aware is the replacement of the fuse. In the event that the MPPI-3 fails to turn on, the line fuse located inside the MPPI should be checked. If the fuse is blown, an exact replacement needs to be used. The MPPI-3 uses a 250mA fast-blow fuse. If the fuse blows again after replacement, please contact ASI for instructions on returning the unit for repair.

2016/03/09 22:12 · asiadmin


end of mg section



Adding a CRISP to a MIM1 makes a MIM2.
Address: 29391 W Enid Rd. Eugene, OR 97402, USA | Phone: +1 (541) 461-8181
playground/mg.txt · Last modified: 2019/09/06 16:24 by vik